simple linear regression equation on phstat

Furthermore, while it is established that lumenal pH affects trafficking, this study revealed a reciprocal link with many mutants defective in anterograde pathways being hyperacidic and retrograde pathway mutants with alkaline vacuoles.

An independent genetic screen revealed that correction of pH v dysregulation in a neo1 ts mutant restored viability whereas cholesterol accumulation in human NPC1 −/− fibroblasts diminished upon treatment with a proton ionophore.

Phospholipid flippases, encoded by the family of P4-type ATPases, emerged as pH regulators, as did the yeast ortholog of Niemann Pick Type C protein, implicated in sterol trafficking. Of 107 mutants that displayed aberrant pH v under more than one external pH condition, functional categories of transporters, membrane biogenesis and trafficking machinery were significantly enriched. Median pH v (5.27☐.13) was resistant to acid stress (5.28☐.14) but shifted significantly in response to alkali stress (5.83☐.13). We undertook a genome-wide survey of vacuole pH (pH v) in 4,606 single-gene deletion mutants of Saccharomyces cerevisiae under control, acid and alkali stress conditions to reveal the vacuolar pH-stat. Despite this fundamental importance, the mechanisms underlying pH homeostasis are not entirely accounted for in any organelle or organism. Transmembrane proton gradients drive ATP synthesis, metabolite transport, receptor recycling and vesicle trafficking, while compartmental pH controls enzyme function. Protons, the smallest and most ubiquitous of ions, are central to physiological processes.

Simple linear regression equation on phstat